Hosuk:LabNotes/2014-1-9: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Hosuki78
No edit summary
>Hosuki78
No edit summary
 
Line 3: Line 3:
===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish===
===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish===
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
====Result ====
====Result ====
*1st Rolony detection probe (Cy5) : count = 5135
*1st Rolony detection probe (Cy5) : count = 5135

Latest revision as of 00:28, 20 January 2014

RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish[edit]

  • PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)


Result[edit]

  • 1st Rolony detection probe (Cy5) : count = 5135
  • RAB7A detection probe (ATTO550) : count = 64
  • Overlap count of RAB7A : count = 14
    • 'Almost no RAB7A target in 1st Rolony…WHY?…