Hosuk:LabNotes/2014-1-9: Difference between revisions
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===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== | ===RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish=== | ||
*PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09) | *PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09) | ||
====Result ==== | ====Result ==== | ||
*1st Rolony detection probe (Cy5) : count = 5135 | *1st Rolony detection probe (Cy5) : count = 5135 |
Latest revision as of 00:28, 20 January 2014
RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish[edit]
- PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)
Result[edit]
- 1st Rolony detection probe (Cy5) : count = 5135
- RAB7A detection probe (ATTO550) : count = 64
- Overlap count of RAB7A : count = 14
- 'Almost no RAB7A target in 1st Rolony…WHY?…