AlanFung:Lab Notebook/DNA Purification: Difference between revisions
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Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added. | Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added. | ||
==Test== | ==Test== | ||
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Revision as of 22:43, 4 August 2013
Date: 10/10/08
== DNeasy Experiment==
Purpose: For purification of total DNA from GC1F fibroblasts cells & PGP1F cells
Material & Protocol:Media:DNeasy.pdf
Notes
-2samples: GC1F and PGP1F(red capped 15mL tube) -Broke down cell pellet by pipetting -Without trypsin EDTA, thawed cells from frozen vials. Start from step 2: lysis on own protocol
Results:
GC1F(Made in Oct.2008) gDNA 36.4ng/uL 260/280: 2.01
PGP1F (Made in Sep. 2008) gDNA: 163.4ng/uL 260/280: 2.08
Both gDNA vials stored in Box 1 in -70freezer located in Kun Lab.
Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added.
Test
sample | Volume |
A | 10 |
B | 20 |