Daniel:Notebook/HiResChrPaint/2014-1-25: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Alu Control (Started 01/08/2014)= Back to Calendar ==Alu Control FISH ([[Daniel:Notebook/HiResC...") |
>Djacobse |
||
Line 4: | Line 4: | ||
==Alu Control FISH ([[Daniel:Notebook/HiResChrPaint/2014-1-24#FISH|Started 01/24/2014]])== | ==Alu Control FISH ([[Daniel:Notebook/HiResChrPaint/2014-1-24#FISH|Started 01/24/2014]])== | ||
''Day 2'' | |||
10. The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 60C for 15 minutes | |||
11. Incubate in 2X SSCT at room temperature for 10 minutes | |||
12. Incubate in 0.2X SSC at room temperature for 10 minutes | |||
13. Wipe gently with kimwipe; do not let the surface containing the cells touch the kimwipe | |||
14. Add 15 uL DRAQ5 to center of dish | |||
15. Cover with 22x22 #1.5 coverslip | |||
16. Seal each slide using nail polish | |||
17. Allow at least 30 minutes for nail polish to dry | |||
18. Stored at 4C ([[Daniel:Notebook/HiResChrPaint/2014-1-27|Microscopy Results]]) |
Latest revision as of 20:19, 27 January 2014
Alu Control (Started 01/08/2014)[edit]
Alu Control FISH (Started 01/24/2014)[edit]
Day 2
10. The next day, remove coverslip carefully and wash slides in pre-warmed 2X SSCT at 60C for 15 minutes 11. Incubate in 2X SSCT at room temperature for 10 minutes 12. Incubate in 0.2X SSC at room temperature for 10 minutes 13. Wipe gently with kimwipe; do not let the surface containing the cells touch the kimwipe 14. Add 15 uL DRAQ5 to center of dish 15. Cover with 22x22 #1.5 coverslip 16. Seal each slide using nail polish 17. Allow at least 30 minutes for nail polish to dry 18. Stored at 4C (Microscopy Results)