Matt:LabNotes/2014-1-31: Difference between revisions
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>Mzcai (Created page with "===Testing=== *Put Shifted in columns 4 and 9 *Put RAB7A_1stRolonyFISH in columns 3 and 10") |
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=== | ===Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells=== | ||
*Previously compared the two dyes in adjacent wells [[Matt:LabNotes/2014-1-28]] | |||
**Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9 | |||
*Put Shifted in columns 4 and 9 | *Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9 | ||
*Put RAB7A_1stRolonyFISH in columns 3 and 10 | *Put RAB7A_1stRolonyFISH in columns 3 and 10 | ||
C9_488: 129 | |||
D9_488: 203 | |||
E9_488: 842 | |||
F9_488: 14,654 (2nd Try) | |||
===RT_RAB7A Primers and the Number of RAB7A Primary Rolonies=== | |||
*Using RAB7A_1stRolonyFISH (ATTO550) | |||
C10_550: 24 | |||
D10_550: 24 | |||
E10_550: 13 | |||
F10_550: 35 |
Revision as of 22:01, 6 February 2014
Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells
- Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
- Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
- Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
- Put RAB7A_1stRolonyFISH in columns 3 and 10
C9_488: 129
D9_488: 203
E9_488: 842
F9_488: 14,654 (2nd Try)
RT_RAB7A Primers and the Number of RAB7A Primary Rolonies
- Using RAB7A_1stRolonyFISH (ATTO550)
C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35