Matt:LabNotes/2014-1-31: Difference between revisions

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(Created page with "===Testing=== *Put Shifted in columns 4 and 9 *Put RAB7A_1stRolonyFISH in columns 3 and 10")
 
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===Testing===
===Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells===
*Previously compared the two dyes in adjacent wells [[Matt:LabNotes/2014-1-28]]
**Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9


*Put Shifted in columns 4 and 9
*Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
*Put RAB7A_1stRolonyFISH in columns 3 and 10
*Put RAB7A_1stRolonyFISH in columns 3 and 10
C9_488: 129
D9_488: 203
E9_488: 842
F9_488: 14,654 (2nd Try)
===RT_RAB7A Primers and the Number of RAB7A Primary Rolonies===
*Using RAB7A_1stRolonyFISH (ATTO550)
C10_550: 24
D10_550: 24
E10_550: 13
F10_550: 35

Revision as of 22:01, 6 February 2014

Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells

  • Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
    • Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
  • Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
  • Put RAB7A_1stRolonyFISH in columns 3 and 10


C9_488: 129 D9_488: 203 E9_488: 842 F9_488: 14,654 (2nd Try)

RT_RAB7A Primers and the Number of RAB7A Primary Rolonies

  • Using RAB7A_1stRolonyFISH (ATTO550)

C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35