Matt:LabNotes/2014-1-31: Difference between revisions
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Well''' | |||
| align="center" style="background:#f0f0f0;"|'''RT primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Cy3''' | |||
| align="center" style="background:#f0f0f0;"|'''Shifted''' | |||
| align="center" style="background:#f0f0f0;"|'''% of Cy3''' | |||
|- | |||
| C9||220nt||711||129||0.181434599 | |||
|- | |||
| D9||300nt||357||203||0.568627451 | |||
|- | |||
| E9||510nt||4449||842||0.189256013 | |||
|- | |||
| F9||1000nt||13295||14654||1.102218879 | |||
|- | |||
| ||||||Average % of Cy3:||0.510384236 | |||
|} | |||
===RT_RAB7A Primers and the Number of RAB7A Primary Rolonies=== | ===RT_RAB7A Primers and the Number of RAB7A Primary Rolonies=== |
Revision as of 22:07, 6 February 2014
Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells
- Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
- Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
- Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
- Put RAB7A_1stRolonyFISH in columns 3 and 10
Well | RT primer | Cy3 | Shifted | % of Cy3 |
C9 | 220nt | 711 | 129 | 0.181434599 |
D9 | 300nt | 357 | 203 | 0.568627451 |
E9 | 510nt | 4449 | 842 | 0.189256013 |
F9 | 1000nt | 13295 | 14654 | 1.102218879 |
Average % of Cy3: | 0.510384236 |
RT_RAB7A Primers and the Number of RAB7A Primary Rolonies
- Using RAB7A_1stRolonyFISH (ATTO550)
C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35