Daniel:Notebook/HiResChrPaint/2014-2-13: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 70: | Line 70: | ||
'''Gel Image''' | '''Gel Image''' | ||
{{GelLanes|Lane3=Low Mass Ladder|Lane4=High MW|Lane5=High MW|Lane6=High MW|Lane7=High MW|Lane8=Low MW|Lane9=Low MW|Lane10=Low MW|Lane12=Low MW}} | |||
[[Image:2013-02-13-HiLoMW.jpg|500px]] | [[Image:2013-02-13-HiLoMW.jpg|500px]] |
Revision as of 22:46, 13 February 2014
Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 02/12/2014)
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results
Nanodrop Results
High MW | A | B | C | D | Low MW | A | B | C | D |
ng/uL | 10.3 | 44.8 | 13.6 | 10.1 | 17.7 | 20.4 | 22.5 | 70.9 | |
ug in 20 uL | 0.2 | 0.9 | 0.3 | 0.2 | 0.4 | 0.4 | 0.5 | 1.4 | |
Total | 1.6 | 2.6 |
Gel Image
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | Low Mass Ladder | High MW | High MW | High MW | High MW | Low MW | Low MW | Low MW | Low MW |