Daniel:Notebook/HiResChrPaint/2014-2-17: Difference between revisions

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>Djacobse
>Djacobse
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'''Nanodrop Results'''
'''Nanodrop Results'''


{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| align="center" width="65" height="15" | &nbsp;
|style="font-weight:bold" width="65" | Low MW A
|style="font-weight:bold" width="65" | Low MW B
|style="font-weight:bold" width="65" | Low MW C
|style="font-weight:bold" width="65" | Low MW D
|style="font-weight:bold" width="65" | HIgh MW A
|style="font-weight:bold" width="65" | HIgh MW B
|style="font-weight:bold" width="65" | HIgh MW C
|style="font-weight:bold" width="65" | HIgh MW D
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 14
| align="center" | 8.1
| align="center" | 21.5
| align="center" | 23.6
| align="center" | 23.6
| align="center" | 0.6
| align="center" | 16.3
| align="center" | 24.8
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 20 uL
| align="center" | 0.3
| align="center" | 0.2
| align="center" | 0.4
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 0.0
| align="center" | 0.3
| align="center" | 0.5
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug total
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 1.3
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | &nbsp;
| align="center" | 1.3
|}


'''Gel Image'''
'''Gel Image'''

Revision as of 18:54, 20 February 2014

Multi-Color FISH (Started 02/08/2014)

Back to Calendar

Cot1 Labeling (Started 2/14/2014)

  1. Measure concentration of all samples in nanodrop
  2. Run on TBE gel to ensure correct size distribution
  3. Pool all high MW samples and all low MW samples

Results

Nanodrop Results

  Low MW A Low MW B Low MW C Low MW D HIgh MW A HIgh MW B HIgh MW C HIgh MW D
ng/uL 14 8.1 21.5 23.6 23.6 0.6 16.3 24.8
ug in 20 uL 0.3 0.2 0.4 0.5 0.5 0.0 0.3 0.5
ug total       1.3       1.3

Gel Image


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample Low Mass Ladder High MW High MW High MW High MW Low MW Low MW Low MW Low MW

File:2014-02-17-Cot1-HiLoMW.jpg