Daniel:Notebook/HiResChrPaint/2014-2-17: Difference between revisions
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'''Nanodrop Results''' | '''Nanodrop Results''' | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt" align="center" | |||
| align="center" width="65" height="15" | | |||
|style="font-weight:bold" width="65" | Low MW A | |||
|style="font-weight:bold" width="65" | Low MW B | |||
|style="font-weight:bold" width="65" | Low MW C | |||
|style="font-weight:bold" width="65" | Low MW D | |||
|style="font-weight:bold" width="65" | HIgh MW A | |||
|style="font-weight:bold" width="65" | HIgh MW B | |||
|style="font-weight:bold" width="65" | HIgh MW C | |||
|style="font-weight:bold" width="65" | HIgh MW D | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ng/uL | |||
| align="center" | 14 | |||
| align="center" | 8.1 | |||
| align="center" | 21.5 | |||
| align="center" | 23.6 | |||
| align="center" | 23.6 | |||
| align="center" | 0.6 | |||
| align="center" | 16.3 | |||
| align="center" | 24.8 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug in 20 uL | |||
| align="center" | 0.3 | |||
| align="center" | 0.2 | |||
| align="center" | 0.4 | |||
| align="center" | 0.5 | |||
| align="center" | 0.5 | |||
| align="center" | 0.0 | |||
| align="center" | 0.3 | |||
| align="center" | 0.5 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug total | |||
| align="center" | | |||
| align="center" | | |||
| align="center" | | |||
| align="center" | 1.3 | |||
| align="center" | | |||
| align="center" | | |||
| align="center" | | |||
| align="center" | 1.3 | |||
|} | |||
'''Gel Image''' | '''Gel Image''' |
Revision as of 18:54, 20 February 2014
Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 2/14/2014)
- Measure concentration of all samples in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results
Nanodrop Results
Low MW A | Low MW B | Low MW C | Low MW D | HIgh MW A | HIgh MW B | HIgh MW C | HIgh MW D | |
ng/uL | 14 | 8.1 | 21.5 | 23.6 | 23.6 | 0.6 | 16.3 | 24.8 |
ug in 20 uL | 0.3 | 0.2 | 0.4 | 0.5 | 0.5 | 0.0 | 0.3 | 0.5 |
ug total | 1.3 | 1.3 |
Gel Image
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | Low Mass Ladder | High MW | High MW | High MW | High MW | Low MW | Low MW | Low MW | Low MW |