Hosuk:LabNotes/2014-2-18: Difference between revisions

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=====Compare prev. method and current method=====
=====Compare prev. method and current method=====
*Different counts and positions for each target
*Different counts and positions for each target
[[File:Compare_Method1-0208_Method2-0217.png|800px]]
[[File:Compare_Method1-0208_Method2-0217.png|900px]]




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*Which one would be correct?...
*Which one would be correct?...


[[File:AnalysisParams_Threshold_SearchRange_4Tests_Counts.png|700px]]
[[File:AnalysisParams_Threshold_SearchRange_4Tests_Counts.png|650px]]
[[File:AnalysisParams_Threshold_SearchRange_4Tests_CompositeImage_Pos2.png|850px]]
[[File:AnalysisParams_Threshold_SearchRange_4Tests_CompositeImage_Pos2.png|900px]]

Revision as of 00:29, 19 February 2014

NCIH1975 : Mutation Detection : Data Analysis

  • Different method from what I've done at 2/9
  • Data from 2/3


Procedure

  • Make one image from all 8 images by Maximum intensity projection , and Run PISA
  • Extract location information from the BW image(--> mask image)
  • Look all 8 images based on the location info from mask image, Decode Rolony signals


  • Set threshold (9000 or 10000), and search range : 9 pixel or 11 pixel (--> 4, or 5 pixel from the center position)
    • Most of rolony signals have above 20000 intensity and backgound have below 7000~8000


Result

Compare prev. method and current method
  • Different counts and positions for each target

File:Compare Method1-0208 Method2-0217.png


Target counts at different parameters
  • Different Threshold and search range values generated different counts as well as different positions...
  • Which one would be correct?...

File:AnalysisParams Threshold SearchRange 4Tests Counts.png File:AnalysisParams Threshold SearchRange 4Tests CompositeImage Pos2.png