Daniel:Notebook/HiResChrPaint/2014-2-16: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Multi-Color FISH (Started 02/08/2014)= Back to Calendar ==Cot1 Labeling ([[Daniel:Notebook/HiRe...") |
>Djacobse No edit summary |
||
Line 11: | Line 11: | ||
#Resuspend each sample in 20 uL nfH2O | #Resuspend each sample in 20 uL nfH2O | ||
##Leave overnight, continued [[Daniel:Notebook/HiResChrPaint/2014-2-17|tomorrow]] | ##Leave overnight, continued [[Daniel:Notebook/HiResChrPaint/2014-2-17|tomorrow]] | ||
[[Category:HRCP]] [[Category:20140208]] [[Category:EtOH Precipitation]] |
Latest revision as of 18:22, 2 July 2014
Multi-Color FISH (Started 02/08/2014)[edit]
Cot1 Labeling (Started 2/14/2014)[edit]
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Leave overnight, continued tomorrow