Matt:LabNotes/2014-2-21: Difference between revisions
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>Mzcai (Created page with "==Screening 32 wells for Ampligase Positive Control Test== *Using 32 wells of 96-well plate Matt:LabNotes/2014-1-24 *Used microscope to check cell density and morphology...") |
>Mzcai mNo edit summary |
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***All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells | ***All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells | ||
[[File:32wellSamples.JPG]] | [[File:32wellSamples.JPG]] | ||
===Mark & Find Pos -> Well=== | |||
*E6 : Pos 1 | |||
*F6 : Pos 2 | |||
*F7 : Pos 3 | |||
*F8 : Pos 4 | |||
*F9 : Pos 5 | |||
*F10 : Pos 6 | |||
*C7 : Pos 7 | |||
*C8 : Pos 8 | |||
*C9 : Pos 9 | |||
*C10 : Pos 10 | |||
*D7 : Pos 11 | |||
*E7 : Pos 12 | |||
===Checking MALAT1 Targets in 1000nt RT_RAB7A=== | ===Checking MALAT1 Targets in 1000nt RT_RAB7A=== | ||
*In wells: F9 and F10 | *In wells: F9 and F10 | ||
*Check with 1uM MALAT1_1stRolonyFISH (Cy3) dye-probe | |||
===Checking for non-specific binding of dcProbe2RevComp-Cy3=== | ===Checking for non-specific binding of dcProbe2RevComp-Cy3=== | ||
*In wells: F7 and F8 | *In wells: F7 and F8 | ||
*Check with 1uM dcProbe2RevComp-Cy3 (ATTO488) dye-probe | |||
===Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies=== | ===Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies=== | ||
*In wells: E6 and F6 | *In wells: E6 and F6 | ||
*Check with 1uM: | |||
**MALAT1_1stRolonyFISH (Cy3) dye-probe | |||
**RAB7A_1stRolonyFISH (ATTO550) dye-probe | |||
**ACTB_1stRolonyFISH (ATTO488) dye-probe |
Revision as of 02:55, 23 February 2014
Screening 32 wells for Ampligase Positive Control Test
- Using 32 wells of 96-well plate Matt:LabNotes/2014-1-24
- Used microscope to check cell density and morphology in each well to pick the best wells for this experiment
- Color coded each well based on quality of cells
- Designated 2 or 4 wells for each sample group
- All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells
Mark & Find Pos -> Well
- E6 : Pos 1
- F6 : Pos 2
- F7 : Pos 3
- F8 : Pos 4
- F9 : Pos 5
- F10 : Pos 6
- C7 : Pos 7
- C8 : Pos 8
- C9 : Pos 9
- C10 : Pos 10
- D7 : Pos 11
- E7 : Pos 12
Checking MALAT1 Targets in 1000nt RT_RAB7A
- In wells: F9 and F10
- Check with 1uM MALAT1_1stRolonyFISH (Cy3) dye-probe
Checking for non-specific binding of dcProbe2RevComp-Cy3
- In wells: F7 and F8
- Check with 1uM dcProbe2RevComp-Cy3 (ATTO488) dye-probe
Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies
- In wells: E6 and F6
- Check with 1uM:
- MALAT1_1stRolonyFISH (Cy3) dye-probe
- RAB7A_1stRolonyFISH (ATTO550) dye-probe
- ACTB_1stRolonyFISH (ATTO488) dye-probe