Song Chen:Daily/NOTES/2014-2-28: Difference between revisions

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>Chensong
(Created page with "===Hepatic maturation from high-density DE differentiation=== Setting: Expose 1000 ms, PFBH 406, 10x obj, <font color=red>ALB</font><b>/</b><font color=green>HNF4A</font> {|{{...")
 
>Chensong
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===Hepatic maturation from high-density DE differentiation===
===Hepatic maturation from high-density DE differentiation===
Setting: Expose 1000 ms, PFBH 406, 10x obj,
Setting: Expose 1000 ms, PFBH 406, 10x obj,
<font color=red>ALB</font><b>/</b><font color=green>HNF4A</font>
<font color=red>ALB</font><b>/</b><font color=green>HNF4A</font>
{|{{table}}
{|{{table}}
|[[File:022814_iPH_ALB.png|300px]]
|[[File:022814_iPH_ALB.jpg|300px]]
|[[File:022814_iPH_HNF4A.png|300px]]
|[[File:022814_iPH_HNF4A.jpg|300px]]
|[[File:022814_iPH_DAPI.png|300px]]
|[[File:022814_iPH_DAPI.jpg|300px]]
|}
|}


<font color=red>CYP3A4</font><b>/</b><font color=green>PROX1</font>
<font color=red>CYP3A4</font><b>/</b><font color=green>PROX1</font>
{|{{table}}
{|{{table}}
|[[File:022814_iPH_3A4.png|300px]]
|[[File:022814_iPH_3A4.jpg|300px]]
|[[File:022814_iPH_PROX1.png|300px]]
|[[File:022814_iPH_PROX1.jpg|300px]]
|[[File:022814_iPH_DAPI.png|300px]]
|[[File:022814_iPH_DAPI.jpg|300px]]
|}
|}



Revision as of 21:58, 28 February 2014

Hepatic maturation from high-density DE differentiation

Setting: Expose 1000 ms, PFBH 406, 10x obj,

ALB/HNF4A

File:022814 iPH ALB.jpg File:022814 iPH HNF4A.jpg File:022814 iPH DAPI.jpg

CYP3A4/PROX1

File:022814 iPH 3A4.jpg File:022814 iPH PROX1.jpg File:022814 iPH DAPI.jpg

iPH(iPS-derived hepatocyte) maturatioin: DF12/B27/HGF, DMEM/ITS

iPH cells express ALB, CYP3A4, while HNF4A is a little bit low.


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