Hosuk:LabNotes/2014-3-3: Difference between revisions

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*Raw: [[Media:MIP_PGP1F-noEGF_Formalin-10_Step1_1stRolony_Laser1-PMT500.tif|1st Rolony]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step5_ACTB_Laser5-PMT680.tif|ACTB]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step7_RAB7A_Laser5-PMT700.tif|RAB7A]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step3_MALAT1_Laser5-PMT700.tif|MALAT1]]
*Raw: [[Media:MIP_PGP1F-noEGF_Formalin-10_Step1_1stRolony_Laser1-PMT500.tif|1st Rolony]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step5_ACTB_Laser5-PMT680.tif|ACTB]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step7_RAB7A_Laser5-PMT700.tif|RAB7A]], [[Media:MIP_PGP1F-noEGF_Formalin-10_Step3_MALAT1_Laser5-PMT700.tif|MALAT1]]
[[File:Rolonies_Fomalin10_ConfocalImage_2014-03-04.png|800px]]
[[File:Rolonies_Fomalin10_ConfocalImage_2014-03-04.png|800px]]
*Comparison
[[File:Counts_Formalin4-10_2014-03-04.png|400px]]

Revision as of 00:12, 5 March 2014

PGP1F cultured with noEGF media : Result

  • Continued from 02/26
  • Detection – 1st Rolonies, ACTB, RAB7A and MALAT1 on 1sdt Rolonies


Result : Sample fixed with 4% Formalin

  • More mRNAs than before, as well as more 1st Rolonies
  • RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes


Counts

File:Counts 2014-03-03.png

  • Counting was done after aligning


Images

File:Rolonies ConfocalImage 2014-03-03.png


Result : Sample fixed with 10% Formalin

  • More mRNAs than before, but fewer than the sample fixed with 4% Formalin
  • More even distribution, not localized at nucleus


Counts

File:Counts Formalin10 2014-03-04.png

  • Counting was done after aligning


Images

File:Rolonies Fomalin10 ConfocalImage 2014-03-04.png


  • Comparison

File:Counts Formalin4-10 2014-03-04.png