Hosuk:LabNotes/2014-3-3: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 No edit summary |
>Hosuki78 No edit summary |
||
Line 39: | Line 39: | ||
*Comparison | *Comparison | ||
[[File:Counts_Formalin4-10_2014-03-04.png| | [[File:Counts_Formalin4-10_2014-03-04.png|500px]] |
Latest revision as of 00:13, 5 March 2014
PGP1F cultured with noEGF media : Result[edit]
- Continued from 02/26
- Detection – 1st Rolonies, ACTB, RAB7A and MALAT1 on 1sdt Rolonies
Result : Sample fixed with 4% Formalin[edit]
- More mRNAs than before, as well as more 1st Rolonies
- RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes
Counts[edit]
- Counting was done after aligning
Images[edit]
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1
File:Rolonies ConfocalImage 2014-03-03.png
Result : Sample fixed with 10% Formalin[edit]
- More mRNAs than before, but fewer than the sample fixed with 4% Formalin
- More even distribution, not localized at nucleus
Counts[edit]
File:Counts Formalin10 2014-03-04.png
- Counting was done after aligning
Images[edit]
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1
File:Rolonies Fomalin10 ConfocalImage 2014-03-04.png
- Comparison