Song Chen:Daily/NOTES/2014-3-6: Difference between revisions

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>Chensong
(Created page with "<b>hES change medium</b>: #PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively; #H9 p62 2*6 cm+24 well*48-well plate in NHSM #H9 Bb6 2...")
 
>Chensong
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|[[File:022614_NHSM_OCT4.png|300px]]
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Revision as of 21:28, 7 March 2014

hES change medium:

  1. PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
  2. H9 p62 2*6 cm+24 well*48-well plate in NHSM
  3. H9 Bb6 2 well*6 well plate
  4. PGPs iPS LCB+CRE p62 1*6 cm in hES medium

send H9 in NHSM:

1*T25 flask


DE differentition(H9 p62, for methylation analysis-Dinh):

3 well*6-well plate, hES at d2, ActA100 d4

check default GFP expression in knockin iPS lines: Setting: Expose 1209 ms, Cerc, 20x obj,

File:022614 NHSM NANOG.png File:022614 NHSM SOX2.png File:022614 NHSM DAPI.png File:022614 NHSM OCT4.png File:022614 NHSM SOX2.2.png File:022614 NHSM DAPI.2.png