Song Chen:Daily/NOTES/2014-3-6: Difference between revisions
Jump to navigation
Jump to search
>Chensong (Created page with "<b>hES change medium</b>: #PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively; #H9 p62 2*6 cm+24 well*48-well plate in NHSM #H9 Bb6 2...") |
>Chensong No edit summary |
||
Line 19: | Line 19: | ||
|[[File:022614_NHSM_SOX2.png|300px]] | |[[File:022614_NHSM_SOX2.png|300px]] | ||
|[[File:022614_NHSM_DAPI.png|300px]] | |[[File:022614_NHSM_DAPI.png|300px]] | ||
| | |||
| | | | ||
|[[File:022614_NHSM_OCT4.png|300px]] | |[[File:022614_NHSM_OCT4.png|300px]] |
Revision as of 21:28, 7 March 2014
hES change medium:
- PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
- H9 p62 2*6 cm+24 well*48-well plate in NHSM
- H9 Bb6 2 well*6 well plate
- PGPs iPS LCB+CRE p62 1*6 cm in hES medium
send H9 in NHSM:
- 1*T25 flask
DE differentition(H9 p62, for methylation analysis-Dinh):
- 3 well*6-well plate, hES at d2, ActA100 d4
check default GFP expression in knockin iPS lines: Setting: Expose 1209 ms, Cerc, 20x obj,
File:022614 NHSM NANOG.png | File:022614 NHSM SOX2.png | File:022614 NHSM DAPI.png | File:022614 NHSM OCT4.png | File:022614 NHSM SOX2.2.png | File:022614 NHSM DAPI.2.png |