Hosuk:LabNotes/2014-3-13: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Mutation Detection with Mixed with PGP1F and NCIH1975 : Ampligase, 2nd RCA=== *Continied from 03/06 *8 Ampligase...") |
>Hosuki78 mNo edit summary |
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*4 NTC(no Ampligase emzyme) | *4 NTC(no Ampligase emzyme) | ||
[[File:Mix_PGP1F_NCIH1975_24wellPlates_AmpligaseMap.png| | [[File:Mix_PGP1F_NCIH1975_24wellPlates_AmpligaseMap.png|300px]] | ||
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*#Run RCA reaction for 24hr. (03/14 ~ 03/15) | *#Run RCA reaction for 24hr. (03/14 ~ 03/15) | ||
*#Anneal dcProbes, and Image | *#Anneal dcProbes, and Image | ||
*Ampligase Mix | |||
**10x Buffer 20uL | |||
**Ampligase (5U/uL) 8uL | |||
**ppEGRF_T790M_WT 2uL | |||
**ppEGRF_T790M_MU 2uL | |||
**ppEGRF_L858R_WT 2uL | |||
**ppEGRF_L858R_MU 2uL | |||
**H2O 164 uL | |||
*NTC Mix | |||
**10x Buffer 20uL | |||
**Ampligase (5U/uL) 0uL | |||
**ppEGRF_T790M_WT 2uL | |||
**ppEGRF_T790M_MU 2uL | |||
**ppEGRF_L858R_WT 2uL | |||
**ppEGRF_L858R_MU 2uL | |||
**H2O 172 uL |
Revision as of 18:08, 13 March 2014
Mutation Detection with Mixed with PGP1F and NCIH1975 : Ampligase, 2nd RCA
- Continied from 03/06
- 8 Ampligase reaction,
- 4 NTC(no Ampligase emzyme)
File:Mix PGP1F NCIH1975 24wellPlates AmpligaseMap.png
- Procedure
- Make Ampligase mix, add
- Incubate at 45C for 24hr. (03/13 ~ 03/14)
- Run RCA reaction for 24hr. (03/14 ~ 03/15)
- Anneal dcProbes, and Image
- Ampligase Mix
- 10x Buffer 20uL
- Ampligase (5U/uL) 8uL
- ppEGRF_T790M_WT 2uL
- ppEGRF_T790M_MU 2uL
- ppEGRF_L858R_WT 2uL
- ppEGRF_L858R_MU 2uL
- H2O 164 uL
- NTC Mix
- 10x Buffer 20uL
- Ampligase (5U/uL) 0uL
- ppEGRF_T790M_WT 2uL
- ppEGRF_T790M_MU 2uL
- ppEGRF_L858R_WT 2uL
- ppEGRF_L858R_MU 2uL
- H2O 172 uL