EricChu:LabNotesMDA/2014-3-6: Difference between revisions
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[[File:03062014NoPolAlu.jpg]] | [[File:03062014NoPolAlu.jpg]] | ||
[[File:03062014NoPolMito.jpg]] | [[File:03062014NoPolMito.jpg]] | ||
* Readings of Alu and Mito | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Chamber''' | |||
| align="center" style="background:#f0f0f0;"|'''Alu (pg)''' | |||
| align="center" style="background:#f0f0f0;"|'''Mito (pg)''' | |||
| align="center" style="background:#f0f0f0;"|'''Mito/Alu''' | |||
|- | |||
| 1||7,430.00||1,166.00||0.16 | |||
|- | |||
| 2||8,796.00||2,686.00||0.31 | |||
|- | |||
| 3||8,138.00||1,684.00||0.21 | |||
|- | |||
| 4||5,504.00||193.10||0.04 | |||
|- | |||
| 5||664.90||54.04||0.08 | |||
|- | |||
| 6||2,206.00||6,943.00||3.15 | |||
|- | |||
| 7||2,029.00||316.20||0.16 | |||
|- | |||
| 8||903.10||46.92||0.05 | |||
|- | |||
| 9||2,633.00||4,187.00||1.59 | |||
|- | |||
| 10||252.30||110.00||0.44 | |||
|- | |||
| 11||12,900.00||4,934.00||0.38 | |||
|- | |||
| 12||3,618.00||899.80||0.25 | |||
|- | |||
| 13||8,731.00||11,010.00||1.26 | |||
|- | |||
| 14||6,054.00||5,821.00||0.96 | |||
|- | |||
| 15||277.70||125.50||0.45 | |||
|- | |||
| 16||4,136.00||4,347.00||1.05 | |||
|- | |||
| 17||9,120.00||6,873.00||0.75 | |||
|- | |||
| 18||18,680.00||15,190.00||0.81 | |||
|- | |||
| 19||7,098.00||36,780.00||5.18 | |||
|- | |||
| 20||4,906.00||13,320.00||2.72 | |||
|- | |||
| 21||10,090.00||10,210.00||1.01 | |||
|- | |||
| 22||7,566.00||18,280.00||2.42 | |||
|- | |||
| 23||6,179.00||11,230.00||1.82 | |||
|- | |||
| 24||5,778.00||2,838.00||0.49 | |||
|- | |||
| 25||15,100.00||1,791.00||0.12 | |||
|- | |||
| Neg Ctrl||5,068.00||95.09||0.02 | |||
|- | |||
| | |||
|} | |||
* The samples were pooled into 3 libraries based on values in Alu. | |||
* Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15) | |||
* Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control) | |||
* Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25) |
Latest revision as of 21:16, 17 March 2014
PGP1#22 Tagmentation without Pol1[edit]
- finished bead purification of No Pol1 samples
- gel image of each sample
- Left to right: Sample 1-10
- Left to right: Sample 11-20
- Right to left: Sample 21-25 and neg control
- qPCR of Alu and Mito
File:03062014NoPolAlu.jpg File:03062014NoPolMito.jpg
- Readings of Alu and Mito
Chamber | Alu (pg) | Mito (pg) | Mito/Alu |
1 | 7,430.00 | 1,166.00 | 0.16 |
2 | 8,796.00 | 2,686.00 | 0.31 |
3 | 8,138.00 | 1,684.00 | 0.21 |
4 | 5,504.00 | 193.10 | 0.04 |
5 | 664.90 | 54.04 | 0.08 |
6 | 2,206.00 | 6,943.00 | 3.15 |
7 | 2,029.00 | 316.20 | 0.16 |
8 | 903.10 | 46.92 | 0.05 |
9 | 2,633.00 | 4,187.00 | 1.59 |
10 | 252.30 | 110.00 | 0.44 |
11 | 12,900.00 | 4,934.00 | 0.38 |
12 | 3,618.00 | 899.80 | 0.25 |
13 | 8,731.00 | 11,010.00 | 1.26 |
14 | 6,054.00 | 5,821.00 | 0.96 |
15 | 277.70 | 125.50 | 0.45 |
16 | 4,136.00 | 4,347.00 | 1.05 |
17 | 9,120.00 | 6,873.00 | 0.75 |
18 | 18,680.00 | 15,190.00 | 0.81 |
19 | 7,098.00 | 36,780.00 | 5.18 |
20 | 4,906.00 | 13,320.00 | 2.72 |
21 | 10,090.00 | 10,210.00 | 1.01 |
22 | 7,566.00 | 18,280.00 | 2.42 |
23 | 6,179.00 | 11,230.00 | 1.82 |
24 | 5,778.00 | 2,838.00 | 0.49 |
25 | 15,100.00 | 1,791.00 | 0.12 |
Neg Ctrl | 5,068.00 | 95.09 | 0.02 |
- The samples were pooled into 3 libraries based on values in Alu.
- Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15)
- Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control)
- Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25)