EricChu:LabNotesMDA/2014-3-6: Difference between revisions

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Line 11: Line 11:
[[File:03062014NoPolAlu.jpg]]
[[File:03062014NoPolAlu.jpg]]
[[File:03062014NoPolMito.jpg]]
[[File:03062014NoPolMito.jpg]]
* Readings of Alu and Mito
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber'''
| align="center" style="background:#f0f0f0;"|'''Alu (pg)'''
| align="center" style="background:#f0f0f0;"|'''Mito (pg)'''
| align="center" style="background:#f0f0f0;"|'''Mito/Alu'''
|-
| 1||7,430.00||1,166.00||0.16
|-
| 2||8,796.00||2,686.00||0.31
|-
| 3||8,138.00||1,684.00||0.21
|-
| 4||5,504.00||193.10||0.04
|-
| 5||664.90||54.04||0.08
|-
| 6||2,206.00||6,943.00||3.15
|-
| 7||2,029.00||316.20||0.16
|-
| 8||903.10||46.92||0.05
|-
| 9||2,633.00||4,187.00||1.59
|-
| 10||252.30||110.00||0.44
|-
| 11||12,900.00||4,934.00||0.38
|-
| 12||3,618.00||899.80||0.25
|-
| 13||8,731.00||11,010.00||1.26
|-
| 14||6,054.00||5,821.00||0.96
|-
| 15||277.70||125.50||0.45
|-
| 16||4,136.00||4,347.00||1.05
|-
| 17||9,120.00||6,873.00||0.75
|-
| 18||18,680.00||15,190.00||0.81
|-
| 19||7,098.00||36,780.00||5.18
|-
| 20||4,906.00||13,320.00||2.72
|-
| 21||10,090.00||10,210.00||1.01
|-
| 22||7,566.00||18,280.00||2.42
|-
| 23||6,179.00||11,230.00||1.82
|-
| 24||5,778.00||2,838.00||0.49
|-
| 25||15,100.00||1,791.00||0.12
|-
| Neg Ctrl||5,068.00||95.09||0.02
|-
|
|}
* The samples were pooled into 3 libraries based on values in Alu.
* Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15)
* Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control)
* Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25)

Latest revision as of 21:16, 17 March 2014

PGP1#22 Tagmentation without Pol1[edit]

  • finished bead purification of No Pol1 samples
  • gel image of each sample
  • Left to right: Sample 1-10

File:03062014NoPolGel1.jpg

  • Left to right: Sample 11-20

File:03062014NoPolGel2.jpg

  • Right to left: Sample 21-25 and neg control

File:03062014NoPolGel3.jpg

  • qPCR of Alu and Mito

File:03062014NoPolAlu.jpg File:03062014NoPolMito.jpg

  • Readings of Alu and Mito
Chamber Alu (pg) Mito (pg) Mito/Alu
1 7,430.00 1,166.00 0.16
2 8,796.00 2,686.00 0.31
3 8,138.00 1,684.00 0.21
4 5,504.00 193.10 0.04
5 664.90 54.04 0.08
6 2,206.00 6,943.00 3.15
7 2,029.00 316.20 0.16
8 903.10 46.92 0.05
9 2,633.00 4,187.00 1.59
10 252.30 110.00 0.44
11 12,900.00 4,934.00 0.38
12 3,618.00 899.80 0.25
13 8,731.00 11,010.00 1.26
14 6,054.00 5,821.00 0.96
15 277.70 125.50 0.45
16 4,136.00 4,347.00 1.05
17 9,120.00 6,873.00 0.75
18 18,680.00 15,190.00 0.81
19 7,098.00 36,780.00 5.18
20 4,906.00 13,320.00 2.72
21 10,090.00 10,210.00 1.01
22 7,566.00 18,280.00 2.42
23 6,179.00 11,230.00 1.82
24 5,778.00 2,838.00 0.49
25 15,100.00 1,791.00 0.12
Neg Ctrl 5,068.00 95.09 0.02
  • The samples were pooled into 3 libraries based on values in Alu.
  • Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15)
  • Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control)
  • Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25)