EricChu:LabNotesMDA/2014-3-11: Difference between revisions

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>Ericchu
(Created page with "==MDA of SW480 in v8 device (SW480#1)== * all 25 samples were collected in ~2ul volume. * 1ul of each was used for tagmentation and 1ul was for CoRE. * TagPCR looked good * a...")
 
>Ericchu
No edit summary
 
Line 7: Line 7:
[[File:03112014SW480TagPCR1.jpg]]
[[File:03112014SW480TagPCR1.jpg]]
[[File:03112014SW480TagPCR2.jpg]]
[[File:03112014SW480TagPCR2.jpg]]
* Left to Right: sample 1-10
* Right to Left: sample 1-10
[[File:03122014SW480Tag1-10.jpg]]
[[File:03122014SW480Tag1-10.jpg]]
* Left to Right: sample 11-20
* Right to Left: sample 11-20
[[File:03122014SW480Tag11-20.jpg]]
[[File:03122014SW480Tag11-20.jpg]]
* Left to Right: sample 21-25
* Left to Right: sample 21-25
[[File:03122014SW480Tag21-25.jpg]]
[[File:03122014SW480Tag21-25.jpg]]
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber'''
| align="center" style="background:#f0f0f0;"|'''Alu (pg)'''
| align="center" style="background:#f0f0f0;"|'''Mito'''
| align="center" style="background:#f0f0f0;"|'''Mito/Alu'''
|-
| 1||5,401.00||10,790.00||2.00
|-
| 2||7,780.00||9,869.00||1.27
|-
| 3||6,911.00||14,710.00||2.13
|-
| 4||19,710.00||16,900.00||0.86
|-
| 5||4,047.00||13,060.00||3.23
|-
| 6||5,828.00||12,220.00||2.10
|-
| 7||3,922.00||5,754.00||1.47
|-
| 8||3,579.00||8,699.00||2.43
|-
| 9||6,954.00||17,970.00||2.58
|-
| 10||8,782.00||49,420.00||5.63
|-
| 11||6,749.00||20,500.00||3.04
|-
| 12||7,200.00||14,400.00||2.00
|-
| 13||8,074.00||9,877.00||1.22
|-
| 14||6,369.00||14,470.00||2.27
|-
| 15||6,332.00||4,257.00||0.67
|-
| 16||11,680.00||11,180.00||0.96
|-
| 17||5,082.00||13,160.00||2.59
|-
| 18||6,241.00||13,070.00||2.09
|-
| 19||9,643.00||22,210.00||2.30
|-
| 20||6,958.00||8,245.00||1.18
|-
| 21||3,720.00||7,207.00||1.94
|-
| 22||6,591.00||11,000.00||1.67
|-
| 23||6,162.00||15,650.00||2.54
|-
| 24||1,666.00||9,499.00||5.70
|-
| 25||616.40||782.80||1.27
|-
| 26||N/A||N/A||#VALUE!
|-
|
|}

Latest revision as of 22:43, 17 March 2014

MDA of SW480 in v8 device (SW480#1)[edit]

  • all 25 samples were collected in ~2ul volume.
  • 1ul of each was used for tagmentation and 1ul was for CoRE.
  • TagPCR looked good
  • all sample pooled together --> EC SW480#1 Tag Mar12

File:03112014SW480TagPCR1.jpg File:03112014SW480TagPCR2.jpg

  • Right to Left: sample 1-10

File:03122014SW480Tag1-10.jpg

  • Right to Left: sample 11-20

File:03122014SW480Tag11-20.jpg

  • Left to Right: sample 21-25

File:03122014SW480Tag21-25.jpg

Chamber Alu (pg) Mito Mito/Alu
1 5,401.00 10,790.00 2.00
2 7,780.00 9,869.00 1.27
3 6,911.00 14,710.00 2.13
4 19,710.00 16,900.00 0.86
5 4,047.00 13,060.00 3.23
6 5,828.00 12,220.00 2.10
7 3,922.00 5,754.00 1.47
8 3,579.00 8,699.00 2.43
9 6,954.00 17,970.00 2.58
10 8,782.00 49,420.00 5.63
11 6,749.00 20,500.00 3.04
12 7,200.00 14,400.00 2.00
13 8,074.00 9,877.00 1.22
14 6,369.00 14,470.00 2.27
15 6,332.00 4,257.00 0.67
16 11,680.00 11,180.00 0.96
17 5,082.00 13,160.00 2.59
18 6,241.00 13,070.00 2.09
19 9,643.00 22,210.00 2.30
20 6,958.00 8,245.00 1.18
21 3,720.00 7,207.00 1.94
22 6,591.00 11,000.00 1.67
23 6,162.00 15,650.00 2.54
24 1,666.00 9,499.00 5.70
25 616.40 782.80 1.27
26 N/A N/A #VALUE!