Dinh/Dinh 2014/NOTES/2014-3-11: Difference between revisions
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= HOTSPOTS 460K Capture = | = HOTSPOTS 460K Capture = | ||
=== Capture calculations === | |||
{| class="wikitable" cellpadding=1 | {| class="wikitable" cellpadding=1 | ||
| align="center" style="background:#f0f0f0;"|'''Size of Probes''' | | align="center" style="background:#f0f0f0;"|'''Size of Probes''' | ||
| align="center" style="background:#f0f0f0;"|'''90000''' | | align="center" style="background:#f0f0f0;"|'''90000''' | ||
Line 34: | Line 34: | ||
| Amount Probes needed||1.69||ng | | Amount Probes needed||1.69||ng | ||
|} | |} | ||
Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp)+157. | Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp) + 157.9 gmoles<sup>-1</sup> | ||
1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup> | 1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup> | ||
Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required) | Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required) | ||
=== Prepared probes === | |||
{| class="wikitable" cellpadding=1 | |||
| align="center" style="background:#f0f0f0;"|'''Subset''' | |||
| align="center" style="background:#f0f0f0;"|'''Dilution''' | |||
| align="center" style="background:#f0f0f0;"|'''QUBIT(ug/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Conc(ng/uL)''' | |||
|- | |||
| A||100||81.2||8.12 | |||
|- | |||
| B||100||46.4||4.64 | |||
|- | |||
| C||100||84.8||8.48 | |||
|- | |||
| D||100||113||11.3 | |||
|- | |||
| E||100||8.47||8.47 | |||
|- | |||
| F||100||97.4||9.74 | |||
|} |
Revision as of 16:50, 19 March 2014
HOTSPOTS 460K Capture
Capture calculations
Size of Probes | 90000 | units |
gDNA | 150 | ng |
Human (MW) | 1.82E+012 | g/moles |
1 Probe (MW) | 31360.1 | g/moles |
Template | 8.23E-011 | nmoles |
Probes(100:1) | 8.23E-009 | nmoles |
Amount Probes needed | 23.23 | ng |
Size of Probes | 6553 | units |
gDNA | 150 | ng |
Human (MW) | 1.82E+012 | g/moles |
1 Probe (MW) | 31360.1 | g/moles |
Template | 8.23E-011 | nmoles |
Probes(100:1) | 8.23E-009 | nmoles |
Amount Probes needed | 1.69 | ng |
Human MW = (3x109 bp)*(607.4 gmoles-1/bp) + 157.9 gmoles-1 1 probe MW = (103 bp)*(303.7 gmoles-1)+ 79 gmoles-1 Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)
Prepared probes
Subset | Dilution | QUBIT(ug/mL) | Conc(ng/uL) |
A | 100 | 81.2 | 8.12 |
B | 100 | 46.4 | 4.64 |
C | 100 | 84.8 | 8.48 |
D | 100 | 113 | 11.3 |
E | 100 | 8.47 | 8.47 |
F | 100 | 97.4 | 9.74 |