Dinh/Dinh 2014/NOTES/2014-3-11: Difference between revisions

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= HOTSPOTS 460K Capture =
= HOTSPOTS 460K Capture =
* Capture calculations
=== Capture calculations ===
{| class="wikitable" cellpadding=1 style="align:center"
{| class="wikitable" cellpadding=1  
| align="center" style="background:#f0f0f0;"|'''Size of Probes'''
| align="center" style="background:#f0f0f0;"|'''Size of Probes'''
| align="center" style="background:#f0f0f0;"|'''90000'''
| align="center" style="background:#f0f0f0;"|'''90000'''
Line 34: Line 34:
| Amount Probes needed||1.69||ng
| Amount Probes needed||1.69||ng
|}
|}
   Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp)+157.9gmoles<sup>-1</sup>
   Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp) + 157.9 gmoles<sup>-1</sup>
   1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup>
   1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup>
   Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)
   Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)
=== Prepared probes ===
{| class="wikitable" cellpadding=1
| align="center" style="background:#f0f0f0;"|'''Subset'''
| align="center" style="background:#f0f0f0;"|'''Dilution'''
| align="center" style="background:#f0f0f0;"|'''QUBIT(ug/mL)'''
| align="center" style="background:#f0f0f0;"|'''Conc(ng/uL)'''
|-
| A||100||81.2||8.12
|-
| B||100||46.4||4.64
|-
| C||100||84.8||8.48
|-
| D||100||113||11.3
|-
| E||100||8.47||8.47
|-
| F||100||97.4||9.74
|}

Revision as of 16:50, 19 March 2014

HOTSPOTS 460K Capture

Capture calculations

Size of Probes 90000 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 23.23 ng
Size of Probes 6553 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 1.69 ng
 Human MW = (3x109 bp)*(607.4 gmoles-1/bp) + 157.9 gmoles-1
 1 probe MW = (103 bp)*(303.7 gmoles-1)+ 79 gmoles-1
 Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)

Prepared probes

Subset Dilution QUBIT(ug/mL) Conc(ng/uL)
A 100 81.2 8.12
B 100 46.4 4.64
C 100 84.8 8.48
D 100 113 11.3
E 100 8.47 8.47
F 100 97.4 9.74