Dinh/Dinh 2014/NOTES/2014-3-11: Difference between revisions

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Revision as of 16:51, 19 March 2014

HOTSPOTS 460K Capture

Capture calculations

Size of Probes 90000 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 23.23 ng
Size of Probes 6553 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 1.69 ng
 Human MW = (3x109 bp)*(607.4 gmoles-1/bp) + 157.9 gmoles-1
 1 probe MW = (103 bp)*(303.7 gmoles-1)+ 79 gmoles-1
 Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)

Prepared probes

Subset Dilution QUBIT(ng/mL) Conc(ng/uL)
A 100 81.2 8.12
B 100 46.4 4.64
C 100 84.8 8.48
D 100 113 11.3
E 100 84.7 8.47
F 100 97.4 9.74