Dinh/Dinh 2014/NOTES/2014-4-8: Difference between revisions

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= HOTSPOTS 460K sequencing library =
= HOTSPOTS 460K sequencing library =
* Indx 28, Indx 29 gel cut:
* Indx 28, Indx 29 gel cut: (Indx28 and 29 are only captured using subset C)
   [[File:HOTSPOTS_Indx28,Indx29_sizeselect.jpg | 300px]]
   [[File:HOTSPOTS_Indx28,Indx29_sizeselect.jpg | 300px]]
* Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
* Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
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* Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
* Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
   450*0.20 + 510*0.80 = 498 bp is the average library size.
   450*0.20 + 510*0.80 = 498 bp is the average library size.
* Library name: '''DD-BSPP_HOTSPOTS-GM12878-Apr9'''
* TBE Gel:
* TBE Gel:
   [[File:HOTSPOTS_seqLib_Apr9.png]]
   [[File:HOTSPOTS_seqLib_Apr9.png]]

Latest revision as of 17:24, 10 April 2014

HOTSPOTS 460K sequencing library[edit]

  • Indx 28, Indx 29 gel cut: (Indx28 and 29 are only captured using subset C)
 File:HOTSPOTS Indx28,Indx29 sizeselect.jpg
  • Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
  • Pool sequencing library together, concentration is ~2.64 ng/ul
  • Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
  450*0.20 + 510*0.80 = 498 bp is the average library size.
  • Library name: DD-BSPP_HOTSPOTS-GM12878-Apr9
  • TBE Gel:
 File:HOTSPOTS seqLib Apr9.png