Hosuk:LabNotes/2014-4-10: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Cancer Cell : Mutation detection test - 2nd Try=== *NCIH-1975 in 24 well plate *Fix : 04/08 *Finish all process 04/10 *Well 1, 2 : EGFR, BR...") |
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[[File:24Well_2014-04-10.png| | [[File:24Well_2014-04-10.png|250px]] | ||
Revision as of 20:58, 10 April 2014
Cancer Cell : Mutation detection test - 2nd Try
- NCIH-1975 in 24 well plate
- Fix : 04/08
- Finish all process 04/10
- Well 1, 2 : EGFR, BRAF RT Primers
- Well 3, 4 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
- Well 5, 6 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
- Well 7, 8, 9, 10 : random hexamer
Result for 1st Rolonies
- Well 1 ~ Well 6 have similar number of rolonies,
- which the numbers are much less than Well 7, 8, and 9.
- (Well 10 has few, it’s because of mistake at RNaseH step)