Blue:RNA-Seq Experiments:04302014: Difference between revisions
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>B1lake (Created page with "== Whole Sort and Tissue Control Libraries == '''RNA Extractions''' *Samples: **20140213 (BA8) - Tissue Chunk **20140213 (BA8) - Bulk Sort 100K **20140410 (BA17) - Tissue Ch...") |
>B1lake |
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*RNA extracted with Zymo ZR RNA Microprep Kit (including DNaseI treatment) | *RNA extracted with Zymo ZR RNA Microprep Kit (including DNaseI treatment) | ||
*RNA concentrations determined using Qubit: | *RNA concentrations determined using Qubit: | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample Concentration (ng/ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Est. RNA/Nuclei (pg)''' | |||
|- | |||
| 20140213 (BA8) - Tissue Chunk||29.3|| | |||
|- | |||
| 20140213 (BA8) - Bulk Sort 100K||7.54||0.754 | |||
|- | |||
| 20140410 (BA17) - Tissue Chunk||55.3|| | |||
|- | |||
| 20140410 (BA17) - Bulk Sort 100K||27.4||2.74 | |||
|- | |||
| 20140415 (BA21) - Tissue Chunk||43.5|| | |||
|- | |||
| 20140415 (BA21) - Bulk Sort 80K||18.8||2.35 | |||
|- | |||
| 20140417 (BA10) - Tissue Chunk||53.5|| | |||
|- | |||
| 20140417 (BA10) - Bulk Sort 100K||14.9||1.49 | |||
|- | |||
| 20140422 (BA22) - Tissue Chunk||63.9|| | |||
|- | |||
| 20140422 (BA22) - Bulk Sort 80K||20.6||2.575 | |||
|- | |||
| 20140424 (BA41/42) - Tissue Chunk||57.3|| | |||
|- | |||
| 20140424 (BA41/42) - Bulk Sort 50K||41.4||8.28 | |||
|- | |||
| | |||
|} |
Latest revision as of 23:09, 1 May 2014
Whole Sort and Tissue Control Libraries[edit]
RNA Extractions
- Samples:
- 20140213 (BA8) - Tissue Chunk
- 20140213 (BA8) - Bulk Sort 100K
- 20140410 (BA17) - Tissue Chunk
- 20140410 (BA17) - Bulk Sort 100K
- 20140415 (BA21) - Tissue Chunk
- 20140415 (BA21) - Bulk Sort 80K
- 20140417 (BA10) - Tissue Chunk
- 20140417 (BA10) - Bulk Sort 100K
- 20140422 (BA22) - Tissue Chunk
- 20140422 (BA22) - Bulk Sort 80K
- 20140424 (BA41/42) - Tissue Chunk
- 20140424 (BA41/42) - Bulk Sort 50K
- RNA extracted with Zymo ZR RNA Microprep Kit (including DNaseI treatment)
- RNA concentrations determined using Qubit:
Sample | Sample Concentration (ng/ul) | Est. RNA/Nuclei (pg) |
20140213 (BA8) - Tissue Chunk | 29.3 | |
20140213 (BA8) - Bulk Sort 100K | 7.54 | 0.754 |
20140410 (BA17) - Tissue Chunk | 55.3 | |
20140410 (BA17) - Bulk Sort 100K | 27.4 | 2.74 |
20140415 (BA21) - Tissue Chunk | 43.5 | |
20140415 (BA21) - Bulk Sort 80K | 18.8 | 2.35 |
20140417 (BA10) - Tissue Chunk | 53.5 | |
20140417 (BA10) - Bulk Sort 100K | 14.9 | 1.49 |
20140422 (BA22) - Tissue Chunk | 63.9 | |
20140422 (BA22) - Bulk Sort 80K | 20.6 | 2.575 |
20140424 (BA41/42) - Tissue Chunk | 57.3 | |
20140424 (BA41/42) - Bulk Sort 50K | 41.4 | 8.28 |