Hosuk:LabNotes/2014-5-23: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Nuclear Staining with DRAQ5=== *DRAQ5 : Excited by 638nm, *Conc. 4uM *Aspirate PBS in MatTek dish, add DRAQ5, incubate for 15min at RT, and i...") |
>Hosuki78 No edit summary |
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====Result==== | ====Result==== | ||
*Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining | *Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining | ||
*[[File:DRAQ5_2014-05-23.png| | *[[File:DRAQ5_2014-05-23.png|1000px]] |
Latest revision as of 20:38, 25 May 2014
Nuclear Staining with DRAQ5[edit]
- DRAQ5 : Excited by 638nm,
- Conc. 4uM
- Aspirate PBS in MatTek dish, add DRAQ5, incubate for 15min at RT, and image without washing step
- Sample : PGP1F (03/21/2014)
Result[edit]
- Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining
- File:DRAQ5 2014-05-23.png