Hosuk:LabNotes/2014-5-23: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===Nuclear Staining with DRAQ5=== *DRAQ5 : Excited by 638nm, *Conc. 4uM *Aspirate PBS in MatTek dish, add DRAQ5, incubate for 15min at RT, and i...")
 
>Hosuki78
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====Result====
====Result====
*Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining
*Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining
*[[File:DRAQ5_2014-05-23.png|1200px]]
*[[File:DRAQ5_2014-05-23.png|1000px]]

Latest revision as of 20:38, 25 May 2014

Nuclear Staining with DRAQ5[edit]

  • DRAQ5 : Excited by 638nm,
  • Conc. 4uM
  • Aspirate PBS in MatTek dish, add DRAQ5, incubate for 15min at RT, and image without washing step
  • Sample : PGP1F (03/21/2014)


Result[edit]