Noi/NOTES/2014-6-3: Difference between revisions
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'''Link to calendar: [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes#2014]]''' | '''Link to calendar: [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes#2014]]''' | ||
* Experimental procedur ewill be added | * Experimental procedur ewill be added | ||
==== PAGE Quantification ==== | |||
* Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel | * Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel | ||
[[File:ZhangLab_2 2014-06-06 08hr 41min_PQ_5ng-CTT-RRBS_4ul.jpg|350px]] | [[File:ZhangLab_2 2014-06-06 08hr 41min_PQ_5ng-CTT-RRBS_4ul.jpg|350px]] | ||
===== PAGE size selection | {| {{table}} class = wikitable | ||
| align="center" style="width:140px;background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="width:80px;background:#f0f0f0;"|'''TruSeq Index''' | |||
| align="center" style="width:80px;background:#f0f0f0;"|'''Conc. (ng/ul)''' | |||
| align="center" style="width:80px;background:#f0f0f0;"|'''Yield in 60ul (ng)''' | |||
| align="center" style="width:80px;background:#f0f0f0;"|'''Amount for 150ng (ul)''' | |||
|- | |||
| CTT-frozen 5ng_1||Ind_9||6.66||399.60||22.52 | |||
|- | |||
| CTT-frozen 5ng_2||Ind_10||15.04||902.62||9.97 | |||
|- | |||
| CTT-FFPE 5ng_1||Ind_11||9.60||575.94||15.63 | |||
|- | |||
| CTT-FFPE 5ng_2||Ind_13||15.09||905.13||9.94 | |||
|} | |||
==== PAGE size selection ==== | |||
* Performed PAGE size selection in 3 wells of 5-well 12% TBE gel | * Performed PAGE size selection in 3 wells of 5-well 12% TBE gel | ||
[[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]] | [[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]] |
Latest revision as of 19:14, 18 June 2014
RRBS, tumor tissue sample (colon)[edit]
Link to calendar: [[1]]
- Experimental procedur ewill be added
PAGE Quantification[edit]
- Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel
File:ZhangLab 2 2014-06-06 08hr 41min PQ 5ng-CTT-RRBS 4ul.jpg
Sample | TruSeq Index | Conc. (ng/ul) | Yield in 60ul (ng) | Amount for 150ng (ul) |
CTT-frozen 5ng_1 | Ind_9 | 6.66 | 399.60 | 22.52 |
CTT-frozen 5ng_2 | Ind_10 | 15.04 | 902.62 | 9.97 |
CTT-FFPE 5ng_1 | Ind_11 | 9.60 | 575.94 | 15.63 |
CTT-FFPE 5ng_2 | Ind_13 | 15.09 | 905.13 | 9.94 |
PAGE size selection[edit]
- Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
File:ZhangLab 2 2014-06-06 10hr 08min 5ng-CTT-RRBS PAGE-SS.jpg
- Verified 2ul of size-selected libraries in 6% TBE gel
File:ZhangLab 2 2014-06-07 22hr 50min PAGE-verify NP-RRBS-CTT-5ng.jpg
Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014
- - Length: 160-360bp
- - Index: Truseq Ind9-11, 13