Daniel:Notebook/HiResChrPaint/2014-6-16: Difference between revisions
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>Djacobse (Created page with "=Probe Design= Back to Calendar Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH pr...") |
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Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control. | Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control. | ||
==Probe Mining== | |||
'''Scripts:''' | |||
[[Media:Oligopaints_Scripts_Manual.pdf|Oligopaints Scripts Manual]] | |||
[[Media:]] | |||
Setting up the scripts for probe mining. I'm doing this locally on my machine for now. | |||
===Setting up a BLAST Database=== | |||
Required for running the Oligopaints scripts. |
Revision as of 22:56, 16 June 2014
Probe Design
Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control.
Probe Mining
Scripts: Oligopaints Scripts Manual [[Media:]]
Setting up the scripts for probe mining. I'm doing this locally on my machine for now.
Setting up a BLAST Database
Required for running the Oligopaints scripts.