Blue:RNA-Seq Experiments:07022014: Difference between revisions
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Diluted to 1ng/ul and used 1ng as tube control for C1 run 20140702 (followed standard Smart+randomer protocol) | Diluted to 1ng/ul and used 1ng as tube control for C1 run 20140702 (followed standard Smart+randomer C1 tube control protocol with 3 fewer cycles in last stage of PCR) | ||
Qubit quantification of PCR product: | |||
#CD4+ 6.56ng/ul | |||
#CD19+ 7.60ng/ul | |||
#PBMC 5.02ng/ul | |||
#Neg Cont 1.64ng/ul |
Revision as of 21:11, 3 July 2014
PBMC C1 Experiment
Extracted RNA from bulk samples using Zymo microRNA Kit and eluted in 6ul. Determined [RNA] using Qubit:
- CD4+ 26.1 ng/uL
- CD19+ 6.27 ng/uL
- PBMC 95.8 ng/uL
Diluted to 1ng/ul and used 1ng as tube control for C1 run 20140702 (followed standard Smart+randomer C1 tube control protocol with 3 fewer cycles in last stage of PCR)
Qubit quantification of PCR product:
- CD4+ 6.56ng/ul
- CD19+ 7.60ng/ul
- PBMC 5.02ng/ul
- Neg Cont 1.64ng/ul