Daniel:Notebook/RNAFISH/2014-7-7: Difference between revisions
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>Djacobse (Created page with "=Probe Design= Back to Calendar Designing probes for Matt and Hosuk's rolony project. The three genes they are testing are ACTB, RAB7A, and MALA...") |
>Djacobse No edit summary |
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#Obtain transcript sequence from [http://genome.ucsc.edu/|UCSC Genome Browser] | #Obtain transcript sequence from [http://genome.ucsc.edu/|UCSC Genome Browser] | ||
##Use genome browser to find genes, and tables to download transcript (mRNA only) | ##Use genome browser to find genes, and tables to download transcript (mRNA only) | ||
# | #Use gene sequence as input for [https://www.biosearchtech.com/stellarisdesigner/|Stellaris probe designer] | ||
##Designer created by Arjun Raj, utilized by Long Cai for paper | |||
##Creates 20mer probes |
Revision as of 21:40, 7 July 2014
Probe Design
Designing probes for Matt and Hosuk's rolony project. The three genes they are testing are ACTB, RAB7A, and MALAT1. I will design probe sets for these three genes.
Workflow
- Obtain transcript sequence from Genome Browser
- Use genome browser to find genes, and tables to download transcript (mRNA only)
- Use gene sequence as input for probe designer
- Designer created by Arjun Raj, utilized by Long Cai for paper
- Creates 20mer probes