Hosuk:LabNotes/2014-7-9: Difference between revisions

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*Rolony with T48 : 30~50% less than rolony with rHex
*Rolony with T48 : 30~50% less than rolony with rHex
*Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
*Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
**Compare to fixing with 10% Formalin at 25C : *[[Hosuk:LabNotes/2014-7-4|LabNote]]
**Compare to fixing with 10% Formalin at 25C : [[Hosuk:LabNotes/2014-7-4|LabNote]]





Revision as of 21:19, 11 July 2014

NCIH1975, PGP1F with T48 RT Primer

  • Fix NCIH1975 and PGP1F with 4% Formalin at 37C
  • S1 : T48 RT primer, S2 : rHexamer

Result

  • Rolony with T48 : 30~50% less than rolony with rHex
  • Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
    • Compare to fixing with 10% Formalin at 25C : LabNote


File:Images PGP1F NCIH1975 20140709 rHex 20x 63x.png


Tissue 11th

  • Tissue 11th: 10um thick, random hexamer, -a-dUTP

Result

  • Result : Tissue 11th: not as many as expected, similar to 9th result


File:Images Tissue 11th 20140709 rHex 20x Pos1 Pos2.png