Blue:RNA-Seq Analyses:C1 hNuc 1000 Dataset: Difference between revisions
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>B1lake |
>B1lake |
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*C1 Run Summary: | *C1 Run Summary: | ||
[[File:20chips_Samplelist.png|400px]] | [[File:20chips_Samplelist.png|400px]] | ||
*Primary Mapping/Analysis done by [ | *Primary Mapping/Analysis done by [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/SingleCell/2014-7-3#Primary_analysis_on_20_chips_of_hNuc_BA8 Rui] using our pipeline | ||
*More in depth analysis by [ | *More in depth analysis by [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/SingleCell/2014-7-4 Rui] | ||
==Average cDNA Yields Across Runs== | ==Average cDNA Yields Across Runs== |
Revision as of 18:20, 11 July 2014
Overview
- C1 Run Summary:
Average cDNA Yields Across Runs
File:20 C1 Runs cDNA Yields 2.png
- Circled experiments represent the same batch of nuclei. There is little effect of freezing the nuclei in 10%DMSO or running the nuclei on a small chip rather than the medium chip. Global levels of cDNA were reduced with adding a nuclei stain/wash step to the loading protocol using the DNA Seq Cell Wash buffer. Also, addition of PolydIdC did globally increase cDNA levels even for the 0_Nuc conditions.