Kun:LabNotes/ExonomeSeq: Difference between revisions

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*[[Kun:LabNotes/ExonomeSeq/2007-11-15]]
*[[Kun:LabNotes/ExonomeSeq/2007-11-15]]
*One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
*One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
==Sequencing library construction==
===Random primed===
*Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN  Tm left = 59.20C
*Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C
===USER-Lig===

Revision as of 01:51, 18 December 2007

<calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar>

Probe Design

  • Kun:LabNotes/ExonomeSeq/2007-11-15
  • One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.

Sequencing library construction

Random primed

  • Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
  • Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C

USER-Lig