Daniel:Notebook/HiResChrPaint/2014-7-21: Difference between revisions
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>Djacobse (Created page with "=Dystrophin Probes (Started 7/18/2014)= Back to Calendar ==Hybridization ([[Daniel:Notebook/HiR...") |
>Djacobse No edit summary |
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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==Hybridization ([[Daniel:Notebook/HiResChrPaint/2014-7-19|Started 7/19/2014)== | ==Hybridization ([[Daniel:Notebook/HiResChrPaint/2014-7-19|Started 7/19/2014]])== | ||
#Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes | #Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes |
Latest revision as of 15:14, 21 July 2014
Dystrophin Probes (Started 7/18/2014)[edit]
Hybridization (Started 7/19/2014)[edit]
- Remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
- Incubate in 2X SSCT at room temperature for 10 minutes
- Incubate in 0.2X SSC at room temperature for 10 minutes
- Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
- Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
- Cover with 22x22 #1.5 coverslip; seal with nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C