Hosuk:LabNotes/2014-7-31: Difference between revisions

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*I guess 1st Rolonies were unfold and gone somehow.
*I guess 1st Rolonies were unfold and gone somehow.
*will try again.
 
 
====Next Run====
*Transfer NCIH1975 to two MatTek dishes (07/31)

Revision as of 21:34, 1 August 2014

Decoding for Mutation detection of Cancer Cell

  • Try decoding (07/30, 07/31)
  • With Two samples
    • S1_0715 : +Ampligase and +KlenTaq at 45C for 24hr.
    • S2_0715 : +Ampligase and +KlenTaq at 60C for 24hr.

Result

  • No signal… need to check again!
  • 1st Rolony signal was not detected.
  • Another sample, ampligase at 60C : also no signal, somehow 1st rolonies were gone…


  • I guess 1st Rolonies were unfold and gone somehow.


Next Run

  • Transfer NCIH1975 to two MatTek dishes (07/31)