Kun:LabNotes/ExonomeSeq: Difference between revisions

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*One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
*One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
*The second probe set: [[Kun:LabNotes/ExonomeSeq/2007-12-21]]
*The second probe set: [[Kun:LabNotes/ExonomeSeq/2007-12-21]]
*Two Agilent 55k libraries were order on 01/04/2008.
#[[Media:Cosmic3760.seq.gz|Cosmic3760]]
#CpgCes: [[Media:Cpg28kV1.txt|Cpg28k]] + [[Media:eSNP27kbg.txt|CES27k]]


==Sequencing library construction==
==Sequencing library construction==

Revision as of 07:28, 5 January 2008

2007 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2007/11/01 view=threemonths </calendar> 2008 <calendar> name=Kun:LabNotes/ExonomeSeq format=%name/%year-%month-%day date=2008/02/01 view=threemonths </calendar>

Probe Design

  • The first probe set: Kun:LabNotes/ExonomeSeq/2007-11-15
  • One 55k library received on 12/12/2007, OLID: 018274110001, 10pmole, resuspended in 500ul RT-PCR grade water (new vial) -> 20nM x 500ul -> Take 100ul out to a 1.5ml non-stick tube as the working tube.
  • The second probe set: Kun:LabNotes/ExonomeSeq/2007-12-21
  • Two Agilent 55k libraries were order on 01/04/2008.
  1. Cosmic3760
  2. CpgCes: Cpg28k + CES27k

Sequencing library construction

Random primed

  • Forward primer (SolFN6):CGACGCTCTTCCGATCTNNNNNN Tm left = 59.20C
  • Reverse primer (SolRN6):CATACGAGCTCTTCCGATCTNNNNNN Tm left = 59.45C

USER-Lig