Matt:LabNotes/2014-8-4: Difference between revisions
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===Column Purification=== | ===Column Purification=== | ||
*Used Zymo ssDNA/RNA columns | *Used Zymo ssDNA/RNA columns | ||
*Total Eluted: ul | *Total Eluted: 124 ul | ||
*Nanodrop quant: ng/ul | *Nanodrop quant: 137.6 ng/ul | ||
*Yield: ng/ul x ul = | *Yield: 137.6 ng/ul x 124 ul = 17.06 ug --> |
Revision as of 21:39, 5 August 2014
Agi26k0gap Probe Production Continued
- Continued from: Matt:LabNotes/2014-7-22
- Thaw and combine the 7 tubes of 200ul that were stored at -20C
TBU Gel Check
- 2ul Sample + 2ul 2X Urea Loading Dye
- 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
- Digested sample
- Undigested sample: 10nM 1st round amplicon
File:2014-08-04 Agi26k0gapDigestCheck.jpg
- Brightest band below 200bp as expected
Column Purification
- Used Zymo ssDNA/RNA columns
- Total Eluted: 124 ul
- Nanodrop quant: 137.6 ng/ul
- Yield: 137.6 ng/ul x 124 ul = 17.06 ug -->