Daniel:Notebook/RNAFISH/2014-8-4: Difference between revisions
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Following my [[Daniel:Notebook/RNAFISH/2014-7-11|previous workflow]] I have generated the probe lists for RBFOX3 and CUX1, which were not in the original list. | Following my [[Daniel:Notebook/RNAFISH/2014-7-11|previous workflow]] I have generated the probe lists for RBFOX3 and CUX1, which were not in the original list. | ||
I ordered the oligos. The plate specs are [[Media:IDTPlate_08042014.txt|here]] (as well as on the spreadsheet). |
Latest revision as of 00:09, 5 August 2014
Probe Design[edit]
Following talks with Dr. Zhang, Rui, and Blue, we're going to push ahead with 4 genes Rui and Blue have identified as the best to try first. Many of them are tissue specific and/or layer specific, and will likely make good controls. They are:
- RBFOX3
- GAD1
- FOXP2
- CUX1
Following my previous workflow I have generated the probe lists for RBFOX3 and CUX1, which were not in the original list.
I ordered the oligos. The plate specs are here (as well as on the spreadsheet).