Daniel:Notebook/HiResChrPaint/2014-8-6: Difference between revisions
>Djacobse |
>Djacobse |
||
Line 4: | Line 4: | ||
==Microscopy== | ==Microscopy== | ||
First I'll test whether the [[Daniel:Notebook/HiResChrPaint/2014-8-5|samples]] can be taken directly to the microscope, with the hybridization buffer still on. This will produce a higher background noise, but will hopefully still result in strong areas of signal distinguishable from the background. Since this is just a test, I'll only use the positive control (sample D, Cot1-488) for now, and I'll investigate the others pending successful trial. | |||
===Sample D=== | |||
<gallery> | <gallery> | ||
Line 9: | Line 13: | ||
File:20140805_Cot1-488_PreWash.lif_Cot1488_2_ch00.jpg|Cells stained with Cot1, Alexa 488 (green), field 2 | File:20140805_Cot1-488_PreWash.lif_Cot1488_2_ch00.jpg|Cells stained with Cot1, Alexa 488 (green), field 2 | ||
</gallery> | </gallery> | ||
As expected, the background is definitely higher, but the signal is also stronger. It will be interesting to do a wash on these samples, and then examine them after. Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples. |
Revision as of 21:29, 6 August 2014
No Wash FISH
Microscopy
First I'll test whether the samples can be taken directly to the microscope, with the hybridization buffer still on. This will produce a higher background noise, but will hopefully still result in strong areas of signal distinguishable from the background. Since this is just a test, I'll only use the positive control (sample D, Cot1-488) for now, and I'll investigate the others pending successful trial.
Sample D
- 20140805 Cot1-488 PreWash.lif Cot1488 1 ch00.jpg
Cells stained with Cot1, Alexa 488 (green), field 1
- 20140805 Cot1-488 PreWash.lif Cot1488 2 ch00.jpg
Cells stained with Cot1, Alexa 488 (green), field 2
As expected, the background is definitely higher, but the signal is also stronger. It will be interesting to do a wash on these samples, and then examine them after. Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.