Sam:LabNotes/Microbione/2009-2-5: Difference between revisions

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>Sam Chiang
>Sam Chiang
Line 11: Line 11:
==Results==
==Results==


'''FIG. Realtime amplification plot (no log in flourescent)'''
'''FIG.1 Realtime amplification plot (no log in flourescent)'''


[[Image:sam020409.jpg]]
[[Image:sam020409.jpg]]
'''FIG.2 Realtime amplification plot (with log in flourescent)'''
[[Image:sam020409-log.jpg]]


==Discussion==
==Discussion==


==Suggestion==
==Suggestion==

Revision as of 19:32, 5 February 2009

Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR

Exp. Design

Template         gDNA      H2O
                --------  ---
Primer: 18S-211  #1   #2   #3    (#1, #2 - replicates)
        18S-306  #4   #5   #6    (#4, #5 - replicates)


Results

FIG.1 Realtime amplification plot (no log in flourescent)

File:Sam020409.jpg


FIG.2 Realtime amplification plot (with log in flourescent)

File:Sam020409-log.jpg

Discussion

Suggestion