Daniel:Notebook/RNAFISH/2014-8-30: Difference between revisions
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>Djacobse (Created page with "=Trial Run (Started 08/29/2014)= Back to Calendar ==Tissue Section Preparation ([[Daniel:Notebokk/RNAFISH/2...") |
>Djacobse |
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[[Daniel:Notebook/RNAFISH|Back to Calendar]] | [[Daniel:Notebook/RNAFISH|Back to Calendar]] | ||
==Tissue Section Preparation ([[Daniel: | ==Tissue Section Preparation ([[Daniel:Notebook/RNAFISH/2014-8-29|From 08/29/2014]])== | ||
#Wash the tissue in PBS, 3 × 30 min | #Wash the tissue in PBS, 3 × 30 min |
Revision as of 18:31, 30 August 2014
Trial Run (Started 08/29/2014)
Tissue Section Preparation (From 08/29/2014)
- Wash the tissue in PBS, 3 × 30 min
- Incubate the tissue in a sucrose series with increasing concentrations:
- 10% for 1 h
- 20% for 1 h
- 30% at 4C overnight