Daniel:Notebook/RNAFISH/2014-8-30: Difference between revisions

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(Created page with "=Trial Run (Started 08/29/2014)= Back to Calendar ==Tissue Section Preparation ([[Daniel:Notebokk/RNAFISH/2...")
 
>Djacobse
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[[Daniel:Notebook/RNAFISH|Back to Calendar]]
[[Daniel:Notebook/RNAFISH|Back to Calendar]]


==Tissue Section Preparation ([[Daniel:Notebokk/RNAFISH/2014-8-29|From 08/29/2014]])==
==Tissue Section Preparation ([[Daniel:Notebook/RNAFISH/2014-8-29|From 08/29/2014]])==


#Wash the tissue in PBS, 3 × 30 min
#Wash the tissue in PBS, 3 × 30 min

Revision as of 18:31, 30 August 2014

Trial Run (Started 08/29/2014)

Back to Calendar

Tissue Section Preparation (From 08/29/2014)

  1. Wash the tissue in PBS, 3 × 30 min
  2. Incubate the tissue in a sucrose series with increasing concentrations:
    1. 10% for 1 h
    2. 20% for 1 h
    3. 30% at 4C overnight