AlanFung:LabNotes/2014/2014-9-8: Difference between revisions

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>Alan6017518
(Created page with "===qPCR Test to verifiy End repair, A-tailing & Adapter Ligation=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconvers...")
 
>Alan6017518
Line 14: Line 14:
| height="14" | Adapter ligated DNA
| height="14" | Adapter ligated DNA
| align="center" | 1
| align="center" | 1
| align="center" | 19.57
| align="center" | 19.6


|-  align="center"
|-  align="center"
| height="14" | 10uM PCR_F
| height="14" | 10uM PCR_F
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 9.785
| align="center" | 9.8


|-  align="center"
|-  align="center"
| height="14" | 10uM PCR_R.Ind2
| height="14" | 10uM PCR_R.Ind2
| align="center" | 0.5
| align="center" | 0.5
| align="center" | 9.785
| align="center" | 9.8


|-  align="center"
|-  align="center"
| height="14" | 2x iQ MM
| height="14" | 2x iQ MM
| align="center" | 12.5
| align="center" | 12.5
| align="center" | 244.625
| align="center" | 244.6


|-  align="center"
|-  align="center"
| height="14" | Water
| height="14" | Water
| align="center" | 10.5
| align="center" | 10.5
| align="center" | 205.485
| align="center" | 205.5


|-  align="center"
|-  align="center"
| height="14" | Total master mix volume
| height="14" | Total master mix volume
| align="center" | 25
| align="center" | 25
| align="center" | 489.25
| align="center" | 489.3


|}
|}

Revision as of 17:15, 8 September 2014

qPCR Test to verifiy End repair, A-tailing & Adapter Ligation

Component 1 Library 19 Libraries + 3%
qPCR Master Mix:    
Adapter ligated DNA 1 19.6
10uM PCR_F 0.5 9.8
10uM PCR_R.Ind2 0.5 9.8
2x iQ MM 12.5 244.6
Water 10.5 205.5
Total master mix volume 25 489.3