Daniel:Notebook/HiResChrPaint/2014-9-9: Difference between revisions
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>Djacobse (Created page with "=Padlock Probes II= Going to retry the padlock probes experiment, using no lambda digestion. ==qPCR== '''Reaction Table''' {| class="wikitable" <hiddentext>generated with...") |
>Djacobse No edit summary |
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#[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]] | #[[Daniel:Protocols/EtOHPrecip|Ethanol Precipitation]] | ||
##Continued [[Daniel:Notebook/HiResChrPaint/2014-9-10|tomorrow]] | ##Continued [[Daniel:Notebook/HiResChrPaint/2014-9-10|tomorrow]] | ||
===qPCR Results=== |
Revision as of 22:54, 9 September 2014
Padlock Probes II
Going to retry the padlock probes experiment, using no lambda digestion.
qPCR
Reaction Table
Component | 1X Reaction | 12.2X Scaled |
Padlock Probes | 0.2 | 2.44 |
100 uM pAP1V41U | 0.4 | 4.88 |
100 uM pAP2V4 | 0.4 | 4.88 |
KAPA SYBR Fast Master Mix (2X) | 50 | 610 |
H2O | 49 | 597.8 |
Total | 100 | 1220 |
- Prepare master mix according to table above
- Add 100 uL reaction to PCR tubes (5 total)
- qPCR Protocol
- 95C 30 sec
- 95C 10 sec
- 55C 20 sec
- 72C 30 sec
- goto (3.2) 17 times
- 72C 2 min
- Hold at 16C forever
- Ethanol Precipitation
- Continued tomorrow