Daniel:Notebook/HiResChrPaint/2014-9-9: Difference between revisions

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=Padlock Probes II=
=Padlock Probes II=
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


Going to retry the padlock probes experiment, using no lambda digestion.  
Going to retry the padlock probes experiment, using no lambda digestion.  

Revision as of 17:51, 10 September 2014

Padlock Probes II

Back to Calendar

Going to retry the padlock probes experiment, using no lambda digestion.

qPCR

Reaction Table

Component 1X Reaction 12.2X Scaled
Padlock Probes 0.2 2.44
100 uM pAP1V41U 0.4 4.88
100 uM pAP2V4 0.4 4.88
KAPA SYBR Fast Master Mix (2X) 50 610
H2O 49 597.8
Total 100 1220
  1. Prepare master mix according to table above
  2. Add 100 uL reaction to PCR tubes (5 total)
  3. qPCR Protocol
    1. 95C 30 sec
    2. 95C 10 sec
    3. 55C 20 sec
    4. 72C 30 sec
    5. goto (3.2) 17 times
    6. 72C 2 min
    7. Hold at 16C forever
  4. Ethanol Precipitation
    1. Continued tomorrow

qPCR Results

File:20140909 PPRL1.png