Daniel:Notebook/HiResChrPaint/2014-9-10: Difference between revisions
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#Set up reaction according to table in a 0.2 mL PCR tube | #Set up reaction according to table in a 0.2 mL PCR tube | ||
#Incubate for 2 hours at 37C | #Incubate for 2 hours at 37C | ||
#Add | #Add 10 uL DpnII buffer (10X) | ||
#Incubate at 94C for 2 minutes | #Incubate at 94C for 2 minutes | ||
#Cool to 37C and incubate for 3 minutes | #Cool to 37C and incubate for 3 minutes |
Revision as of 17:56, 10 September 2014
Padlock Probes II
Nanodrop Results
DpnII/USER Digestion
Reaction Table
Reagent | PPRL (uL) |
amplicon | 30 |
ug Input | 2.0 |
USER | 5 |
nf H2O | 45 |
Total | 80 |
- Set up reaction according to table in a 0.2 mL PCR tube
- Incubate for 2 hours at 37C
- Add 10 uL DpnII buffer (10X)
- Incubate at 94C for 2 minutes
- Cool to 37C and incubate for 3 minutes
- Add 5 uL DpnII and incubate at 37C for 2 hours
- Heat inactivate DpnII at 65C for 20 minutes