Daniel:Notebook/HiResChrPaint/2014-9-10: Difference between revisions

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===Nanodrop Results===
===Nanodrop Results===
[[Category:HRCP]] [[Category:DpnII]] [[Category:USER]] [[Category:20140909]]

Revision as of 18:02, 10 September 2014

Padlock Probes II

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Nanodrop Results

DpnII/USER Digestion

Reaction Table

Reagent PPRL (uL)
amplicon 30
ug Input 2.0
USER 5
nf H2O 45
Total 80
  1. Set up reaction according to table in a 0.2 mL PCR tube
  2. Incubate for 2 hours at 37C
  3. Add 10 uL DpnII buffer (10X)
  4. Incubate at 94C for 2 minutes
  5. Cool to 37C and incubate for 3 minutes
  6. Add 5 uL DpnII and incubate at 37C for 2 hours
  7. Heat inactivate DpnII at 65C for 20 minutes

Nanodrop Results