Daniel:Notebook/HiResChrPaint/2014-9-20: Difference between revisions

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The microscopy for this project was done over several days, so I'm just going to report them on the day I did the microscopy and give a list here.
The microscopy for this project was done over several days, so I'm just going to report them on the day I did the microscopy and give a list here.


*'''GAD1-488 and RBFOX3-594''':[[Daniel:Notebook/HiResChrPaint/2014-9-21|9/21/2014]]
<dynamicpagelist>
category=HRCP
category=Microscopy
category=20140919
<dynamicpagelist>




[[Category:HRCP]] [[Category:Hybridization]] [[Category:Microscopy]] [[Category:20140919]]
[[Category:HRCP]] [[Category:Hybridization]] [[Category:Microscopy]] [[Category:20140919]]

Revision as of 18:45, 24 September 2014

Slide FISH

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Hybridization

  1. Remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes
  2. Incubate slides in 2X SSCT at room temperature for 10 minutes
  3. Incubate slides in 0.2X SSC at room temperature for 10 minutes
  4. Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels
  5. Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip
  6. Invert slides onto coverslips with mounting media
  7. Seal each slide using nail polish
  8. Allow at least 30 minutes for nail polish to dry
  9. Slides are quite stable for several months at 4C

Microscopy

The microscopy for this project was done over several days, so I'm just going to report them on the day I did the microscopy and give a list here.

<dynamicpagelist> category=HRCP category=Microscopy category=20140919 <dynamicpagelist>