Daniel:Notebook/HiResChrPaint/2014-9-20: Difference between revisions
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category=Microscopy | category=Microscopy | ||
category=20140919 | category=20140919 | ||
<dynamicpagelist> | </dynamicpagelist> | ||
[[Category:HRCP]] [[Category:Hybridization]] [[Category:Microscopy]] [[Category:20140919]] | [[Category:HRCP]] [[Category:Hybridization]] [[Category:Microscopy]] [[Category:20140919]] |
Latest revision as of 19:15, 24 September 2014
Slide FISH[edit]
Hybridization[edit]
- Remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes
- Incubate slides in 2X SSCT at room temperature for 10 minutes
- Incubate slides in 0.2X SSC at room temperature for 10 minutes
- Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels
- Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip
- Invert slides onto coverslips with mounting media
- Seal each slide using nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C
Microscopy[edit]
The microscopy for this project was done over several days, so I'm just going to report them on the day I did the microscopy and give a list here.
<dynamicpagelist> category=HRCP category=Microscopy category=20140919 </dynamicpagelist>