Hosuk:LabNotes/2014-9-29: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===Tissue Section : fixing test=== *The reference that Dan uses : fix tissue section for 4h~24h with formaldehyde *Let’s try fixing for longer...")
 
>Hosuki78
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====Procedure====
====Procedure====
*Fix : 2014-09-29, 4% Formaldehyde, 4hr at room temp.
*Fix : 2014-09-29, 4% Formaldehyde, 4hr at room temp.
*RT : 2014-09-29, -a-dUTP --> Tissue remained well
*RT : 2014-09-29, -a-dUTP --> Tissue remained well (continued to 2014-10-01)
*RCA : 2014-09-30, 1pm~ (continued to 2014-10-01)

Revision as of 17:34, 2 October 2014


Tissue Section : fixing test

  • The reference that Dan uses : fix tissue section for 4h~24h with formaldehyde
  • Let’s try fixing for longer time
  • Test with Mouse brain tissue section (now I have only two human brain sample…) fixing for 4hr, 1hr, and compare them!


Procedure

  • Fix : 2014-09-29, 4% Formaldehyde, 4hr at room temp.
  • RT : 2014-09-29, -a-dUTP --> Tissue remained well (continued to 2014-10-01)