Blue:RNA-Seq Experiments:10062014: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>B1lake
>B1lake
 
Line 1: Line 1:
== Size Analysis of Mouse DRG Nuclei ==
== Size Analysis of Mouse DRG Nuclei ==


*Recieved DRG nuclei (p2) from Lulu (Guoping Fan Lab, UCLA) on 10-03-2014
*Recieved DRG nuclei (Mouse P2) from Ganlu (Guoping Fan Lab, UCLA) on 10-03-2014


* Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
* Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
* Checked under microscope - extensive clusters
* Checked under microscope - extensive clusters
* Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
* Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
* Measured nuclei width using microscope (10x objective) and metamorph software:
* Measured nuclei width using microscope (10x objective) and Metamorph software:


{| {{table}} border
{| {{table}} border

Latest revision as of 23:00, 6 October 2014

Size Analysis of Mouse DRG Nuclei[edit]

  • Recieved DRG nuclei (Mouse P2) from Ganlu (Guoping Fan Lab, UCLA) on 10-03-2014
  • Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
  • Checked under microscope - extensive clusters
  • Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
  • Measured nuclei width using microscope (10x objective) and Metamorph software:
Nuclei Width (um)
6.45
9.03
6.45
5.8
9.03
5.8
4.52
7.21
5.95
5.32
7.1
7.1
6.45
5.8
7.1
7.74
5.8
5.8
5.8
5.8
7.1
5.16
5.8
8.48
10.32
7.1
5.95
5.32
5.8
7.1
9.68
6.45
Mean = 6.6971875