Blue:RNA-Seq Experiments:10062014: Difference between revisions
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== Size Analysis of Mouse DRG Nuclei == | == Size Analysis of Mouse DRG Nuclei == | ||
*Recieved DRG nuclei ( | *Recieved DRG nuclei (Mouse P2) from Ganlu (Guoping Fan Lab, UCLA) on 10-03-2014 | ||
* Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um | * Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um | ||
* Checked under microscope - extensive clusters | * Checked under microscope - extensive clusters | ||
* Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul | * Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul | ||
* Measured nuclei width using microscope (10x objective) and | * Measured nuclei width using microscope (10x objective) and Metamorph software: | ||
{| {{table}} border | {| {{table}} border |
Latest revision as of 23:00, 6 October 2014
Size Analysis of Mouse DRG Nuclei[edit]
- Recieved DRG nuclei (Mouse P2) from Ganlu (Guoping Fan Lab, UCLA) on 10-03-2014
- Diluted nuclei to 600/ul, ran on biorad - one tight peak at 5um
- Checked under microscope - extensive clusters
- Filtered with 20um filter to clean up - removed many clusters - dropped [] to 250/ul
- Measured nuclei width using microscope (10x objective) and Metamorph software:
Nuclei Width (um) |
6.45 |
9.03 |
6.45 |
5.8 |
9.03 |
5.8 |
4.52 |
7.21 |
5.95 |
5.32 |
7.1 |
7.1 |
6.45 |
5.8 |
7.1 |
7.74 |
5.8 |
5.8 |
5.8 |
5.8 |
7.1 |
5.16 |
5.8 |
8.48 |
10.32 |
7.1 |
5.95 |
5.32 |
5.8 |
7.1 |
9.68 |
6.45 |
Mean = 6.6971875 |