Daniel:Notebook/HiResChrPaint/2014-11-5: Difference between revisions
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>Djacobse (Created page with "=FISH (Started 11/4/2014) Back to Calendar ==Hybridization== #Remove coverslip carefully and w...") |
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=FISH ([[Daniel:Notebook/HiResChrPaint/2014-11-4|Started 11/4/2014]]) | =FISH ([[Daniel:Notebook/HiResChrPaint/2014-11-4|Started 11/4/2014]])= | ||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Latest revision as of 17:34, 5 November 2014
FISH (Started 11/4/2014)[edit]
Hybridization[edit]
- Remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes
- Incubate slides in 2X SSCT at room temperature for 10 minutes
- Incubate slides in 0.2X SSC at room temperature for 10 minutes
- Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels
- Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip
- Invert slides onto coverslips with mounting media
- Seal each slide using nail polish
- Allow at least 30 minutes for nail polish to dry
- Slides are quite stable for several months at 4C