Daniel:Notebook/Haplotyping/SISSORPipeline: Difference between revisions

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[[EricChu:LabNotesMDA/2014-4-16|Eric's Original Algorithm]]
[[EricChu:LabNotesMDA/2014-4-16|Eric's Original Algorithm]]


==Algorithm==
==Algorithm Overview==


#Python script for establishing bin read counts
#Python script for establishing bin read counts

Revision as of 22:32, 3 December 2014

SISSOR Segmentation Pipeline

Back to Haplotyping Notebook

The purpose of this section is to include data from my recreation/use of Eric's algorithm to properly segment SISSOR data. Since SISSOR has very low coverage, the samtools targetcut function does not properly identify fragments created in the microfluidic device. Another important part of this is to make sure the output is consistent with the samtools targetcut format for easier combination with the BAC/Hi-C data for HAPCUT.

Eric's Original Algorithm

Algorithm Overview

  1. Python script for establishing bin read counts
  2. Cutoff to define initial states--may be a weak point
  3. Hidden Markov Model to smooth initial states to final states
  4. Produce a .sam format matching samtools targetcut--Extra step I need

Calendar

<calendar> name=Daniel:Notebook/Haplotyping/SISSORPipeline format=%name/%year-%month-%day date=2014/02/01 view=oneyear </calendar>