Noi/NOTES/2014-12-25: Difference between revisions
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[[noi:DMR220k_LabNotes#2014| '''Back to calendar''']] | [[noi:DMR220k_LabNotes#2014| '''Back to calendar''']] | ||
== 1) HiSeq Rapid run at IGM (141216_SN1001_0423) == | == 1) HiSeq Rapid run at IGM (141216_SN1001_0423) == | ||
* Library type: WGBS libraries generated by KAPA Hyper Prep kit (all cancer plasma samples). NC samples were generated by original method but lacked T4 DNA Pol, T4 polynucleotide kinase. | |||
* The WGBS libraries were captured by Nimblegen SeqCap LMS probe. | |||
* PE 100+7 | * PE 100+7 | ||
{| {{table}} border = 1 | {| {{table}} border = 1 |
Revision as of 00:08, 26 December 2014
Summary of WGBS sequencing libraries captured by SeqCap Hybridization capture and their corresponding index
1) HiSeq Rapid run at IGM (141216_SN1001_0423)
- Library type: WGBS libraries generated by KAPA Hyper Prep kit (all cancer plasma samples). NC samples were generated by original method but lacked T4 DNA Pol, T4 polynucleotide kinase.
- The WGBS libraries were captured by Nimblegen SeqCap LMS probe.
- PE 100+7
Library ID (for sequencing) | Sample ID | Adaptor System/primers | Index # | Seq (RC) | Sequencing platform/at | Lane # |
NP-SeqCap-pCancerSetB-Dec4 | PC-P_3 | TruSeq | 2 | CGATGT | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | PC-P_5 | TruSeq | 4 | TGACCA | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | PC-P_6 | TruSeq | 5 | ACAGTG | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | PC-P_7 | TruSeq | 6 | GCCAAT | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | PC-P_8 | TruSeq | 7 | CAGATC | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 6-P-4 | TruSeq | 12 | CTTGTA | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 6-P-5 | TruSeq | 13 | AGTCAA | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 6-P-8 | TruSeq | 14 | AGTTCC | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 6-P-9 | TruSeq | 15 | ATGTCA | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 6-P-10 | TruSeq | 16 | CCGTCC | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec4 | 7-P-1 | TruSeq | 18 | GTCCGC | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec15 | 7-P-2 | TruSeq | 19 | GTGAAA | Rapid run/IGM | 1 |
NP-SeqCap-pCancerSetB-Dec16 | NC-29 | TruSeq | 23 | GAGTGG | Rapid run/IGM | 1 |
Library ID (for sequencing) | Sample ID | Adaptor System/primers | Index # | Seq (RC) | Sequencing platform/at | Lane # |
NP-SeqCap-tCancerSetC-Dec4 N2 | 6T-1 | STD ILMN adaptor/PCR_R.N2 Indx | 4 | TGACCA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 6T-2 | STD ILMN adaptor/PCR_R.N2 Indx | 5 | ACAGTG | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 6T-3 | STD ILMN adaptor/PCR_R.N2 Indx | 6 | GCCAAT | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 6T-4 | STD ILMN adaptor/PCR_R.N2 Indx | 7 | CAGATC | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 6T-5 | STD ILMN adaptor/PCR_R.N2 Indx | 8 | ACTTGA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 7T-1 | STD ILMN adaptor/PCR_R.N2 Indx | 9 | GATCAG | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 7T-2 | STD ILMN adaptor/PCR_R.N2 Indx | 10 | TAGCTT | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 7T-4 | STD ILMN adaptor/PCR_R.N2 Indx | 12 | CTTGTA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | 7T-5 | STD ILMN adaptor/PCR_R.N2 Indx | 13 | CCATGA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | PCT-1 | STD ILMN adaptor/PCR_R.N2 Indx | 14 | AAGACA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | PCT-2 | STD ILMN adaptor/PCR_R.N2 Indx | 15 | CTTCCT | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | PCT-4 | STD ILMN adaptor/PCR_R.N2 Indx | 16 | GGGGTT | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | PCT-6 | STD ILMN adaptor/PCR_R.N2 Indx | 17 | CTCATC | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | PCT-7 | STD ILMN adaptor/PCR_R.N2 Indx | 18 | AGTTCA | Rapid run/IGM | 2 |
NP-SeqCap-tCancerSetC-Dec4 N2 | NC-6 | TruSeq | 6 | GCCAAT | Rapid run/IGM | 2 |